Fermentas turbofect manual

coding plasmid and 6 µl of TurboFect in vivoTransfection reagents per mouse. The delivery of the transgene was evaluated by measurement of Firefly luciferase activity in total protein extracted from the lungs of transfected animals. % of testedmice expressed the Firefly luciferase. Thermo Scientific™ ™TurboFect Transfection Reagent (Cat #R, R) is a solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery into eukaryotic cells. TurboFect reagent is ideal for transfectionMissing: fermentas. Turbofect Sirna Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Price from $ to $

TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. coding plasmid and 6 µl of TurboFect in vivoTransfection reagents per mouse. The delivery of the transgene was evaluated by measurement of Firefly luciferase activity in total protein extracted from the lungs of transfected animals. % of testedmice expressed the Firefly luciferase. Essential tools for your molecular biology workflows. Our Thermo Scientific molecular biology products are designed to deliver reproducibility and performance in everyday applications including reverse transcription, PCR, nucleic acid electrophoresis, restriction enzyme digests, and cloning.

Jan cells were transfected using Turbofect (Fermentas). Ultra kit (Ambion) according to the manufacturer's instructions and diluted to. Oct The TurboFect/rNef-Vif-GpP24 and TurboFect/rNef-Vpu-GpP24 Fermentas, Germany) according to the manufacturer's instructions. Lipofectamine (cationic lipid, Invitrogen) and TurboFect (cationic polymer, Fermentas) transfection reagents were employed to deliver pEGFP-Nef into.