Invitrogen pbad manual

Gateway Manual Invitrogen For details about a particular Invitrogen destination vector or pBAD/Thio His TOPO manual the Gateway Manual Invitrogen Printable_ Read any ebook online with basic steps. But if you want to download it to your smartphone, you can download much of ebooks now. Download Now. The pBAD/His Kit includes 20 µg of each pBAD/His A, B, C vector, 20 µg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at °C. Store LMG and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. Cleavage. Life Technologies was acquired by Thermo Fisher Scientific in January, Our innovative life science, applied science, and clinical products are now found under the Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent™, and Molecular Probes™ brands. Home page Shop all products.

Many of the reagents supplied with the pBAD-TOPO® TA Expression Kit and other reagents suitable for use with the kit are available separately from Invitrogen. Ordering information for these reagents is provided below. Product Amount Catalog no. reactions reactions Platinum® Taq DNA Polymerase reactions pBAD/His A, B, and C pBAD/Myc- His A, B, and C Kit contents and storage Contents This manual is supplied with the following kits. Cat. No. Contents V 20 µg each of pBAD/His A, B, and C vector in TE Buffer, pH [1] (40 µl at. Entry Clone To recombine your gene of interest into pBAD-DEST49, you should have an entry clone containing your gene of interest. For your convenience, Invitrogen offers the pENTR Directional TOPO® Cloning Kit (page v) for 5-minute cloning of your gene of interest into an entry vector. For detailed information on constructing an entry clone.

In this study, the ace gene was successfully cloned in pBAD/Thio-TOPO the manual (pBAD/TOPO7 ThioFusion™ Expression System, Version B, Invitrogen, USA. Download pGLO Map and Sequence (PDF) The pGLO plasmid contains both the promoter (pBAD) and araC gene, but araB, araA, and araD have been replaced by. sequence is utilized for secretion of the recombinant protein into the periplasmic Laboratory Manual (Sambrook et al., ) or Current Protocols in.